If you want to insert vertical line between Content & Right Bar in Genesis add below css line in your style.css

.single .entry {
    border-right: solid 1px #eee;
    padding-right: 30px;
}

Add below line for Table & Mobile (For @media 992px and below)

@media only screen and (max-width : 992px){
.single .entry {
    border-right: none;
	padding-right:0px;
}

NOTE: If you used Genesis - Featured Post on the Right bar, you need to add below css

.sidebar-primary .entry {
    border-right: none!important;
	padding-right:0px!important;
}