If you want to insert vertical line between Content & Right Bar in Genesis add below css line in your style.css
.single .entry { border-right: solid 1px #eee; padding-right: 30px; }
Add below line for Table & Mobile (For @media 992px and below)
@media only screen and (max-width : 992px){ .single .entry { border-right: none; padding-right:0px; }
NOTE: If you used Genesis - Featured Post on the Right bar, you need to add below css
.sidebar-primary .entry { border-right: none!important; padding-right:0px!important; }